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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Genética
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JoVE Journal Genética
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

6,169 Views

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08:50 min

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May 14, 2020

DOI:

10.3791/61231-v

DOI:
10.3791/61231-v

•

08:50 min
May 14, 2020

•

8 Views
, , ,
1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School,University of Padua, 3Biologie de l’Ecole Normale Supérieure (IBENS), Paris, France

Summary

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The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification.

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Saplaoura, E., Perrera, V., Colot, V., Kragler, F. Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis. J. Vis. Exp. (159), e61231, doi:10.3791/61231 (2020).

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