Journal
/
Immunology and Infection
/
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

DOI:
10.3791/61178-v

•

05:59 min

•

May 14, 2020

•

, ,
1Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University,Harbin Medical University, 2Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine,University of Calgary

Chapters

  • 00:04Introduction
  • 00:40Preparation of Materials
  • 01:11Preparation of the Animal for Surgery
  • 01:47Surgical Suturing of Afferent Lymphatic Vessels
  • 03:47Tracking the Lymph Flow
  • 04:31Results: Selective Prevention of Lymph Flow
  • 05:09Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is presented.

Tags

Keywords: Lymph FlowLymphatic VesselsAfferent Lymphatic VesselsPopliteal Lymph NodesIntradermal InjectionEvans Blue DyeSutureMouseAnesthesiaMicrosurgery

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61178?article_type=v

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
check_url/61178?article_type=v

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
check_url/61178?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/61178?article_type=v

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
check_url/61178?article_type=v

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
check_url/61178?article_type=v

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
check_url/61178?article_type=v

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
check_url/61178?article_type=v

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
check_url/61178?article_type=v

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry
check_url/61178?article_type=v

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Read Article