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Em Utero Eletroporation de Marcadores de Cor Multiaddressable (MAGIC) para Individualizar Astrócitos de Ratos Cortical
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Neuroscience
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JoVE Journal Neuroscience
In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes

Em Utero Eletroporation de Marcadores de Cor Multiaddressable (MAGIC) para Individualizar Astrócitos de Ratos Cortical

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5,203 Views

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06:47 min

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May 21, 2020

DOI:

10.3791/61110-v

DOI:
10.3791/61110-v

•

06:47 min
May 21, 2020

•

8 Views
, , , , , , , , , ,
1Université de Montpellier, INSERM,Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS,Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM,IP Paris

Summary

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Os astrócitos ladrilho o córtex cerebral uniformemente, tornando a análise de sua morfologia complexa desafiadora a nível celular. O protocolo aqui fornecido usa rotulagem multicolorida baseada na eletroporação uterónica para destacar os astrócitos corticais e analisar seu volume e morfologia com um pipeline de análise de imagem fácil de usar.

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Dumas, L., Clavreul, S., Durand, J., Hernandez-Garzon, E., Abdeladim, L., Barry-Martinet, R., Caballero-Megido, A., Beaurepaire, E., Bonvento, G., Livet, J., Loulier, K. In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes. J. Vis. Exp. (159), e61110, doi:10.3791/61110 (2020).

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