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Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
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Neuroscience
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JoVE Journal Neuroscience
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

English

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9,589 Views

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10:12 min

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May 05, 2020

DOI:

10.3791/60993-v

DOI:
10.3791/60993-v

•

10:12 min
May 05, 2020

•

12 Views
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1Department of Physiology and Pharmacology, Sackler Faculty of Medicine,Tel Aviv University, 2Sagol School of Neuroscience,Tel Aviv University

Summary

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Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

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Cite this Article

Altman, T., Maimon, R., Ionescu, A., Pery, T. G., Perlson, E. Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System. J. Vis. Exp. (159), e60993, doi:10.3791/60993 (2020).

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