ジャーナル
/
神経科学
/
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

DOI:
10.3791/60993-v

•

10:12 min

•

May 05, 2020

•

, , , ,
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine,テルアビブ大学, 2Sagol School of Neuroscience,テルアビブ大学

章

  • 00:04Introduction
  • 01:08Microfluidic Chamber Preparation
  • 05:53Neuronal Culture Plating
  • 07:24Axonal Transport
  • 08:19Results: Axonal Transport of Mitochondria and Acidic Compartments in Motor Neurons
  • 09:34Conclusion

概要

自動翻訳

自動翻訳

Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

タグ

Axonal TransportMotor NeuronMicrofluidic ChambersNeurodegenerative DiseaseALSAxonal GrowthAxonal DegenerationPDMSChlorotrimethylsilaneVacuum Desiccator

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Paired Patch Clamp Recordings from Motor-neuron and Target Skeletal Muscle in Zebrafish

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration

In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Read Article