Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.
Altman, T., Maimon, R., Ionescu, A., Pery, T. G., Perlson, E. Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System. J. Vis. Exp. (159), e60993, doi:10.3791/60993 (2020).