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生成基于人类 iPSC 的血脑屏障芯片
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
DOI:

10:20 min

March 02, 2020

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Chapters

  • 00:05Introduction
  • 01:27Seeding the “Brain Side” Channel and Differentiating EZ Spheres into Mixed Neural Cultures
  • 03:04Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the “Blood Side”
  • 05:27Initiation of Flow
  • 08:12Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip
  • 09:17Conclusion

Summary

Automatic Translation

血脑屏障(BBB)是一种多细胞神经血管单元,严密调节大脑平衡。通过结合人类 iPSC 和芯片上器官技术,我们生成了个性化的 BBB 芯片,适用于疾病建模和 CNS 药物渗透性预测。介绍了BBB芯片的生成和运行的详细方案。

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