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Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

13,822 Views

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10:24 min

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May 24, 2020

DOI:

10.3791/60843-v

DOI:
10.3791/60843-v

•

10:24 min
May 24, 2020

•

4 Views
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1Center for Molecular Medicine,University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

Tags

Human Pluripotent Stem CellsSympathetic NeuronsFeeder-freeChemically DefinedEfficient DifferentiationElectrical ActivityDisease ModelingRegenerative MedicineDrug ScreeningCo-culture System

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Wu, H. F., Zeltner, N. Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions. J. Vis. Exp. (159), e60843, doi:10.3791/60843 (2020).

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