Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Hsueh  Fu Wu; Nadja Zeltner

doi:10.3791/60843

/

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

JoVE Journal
発生生物学
Author Produced

このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。

JoVE Journal 発生生物学

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

13,934 Views

•

10:24 min

•

May 24, 2020

DOI:

10.3791/60843-v

DOI:

10.3791/60843-v

•

10:24 min

May 24, 2020

•

32 Views

Hsueh Fu Wu^1,2, Nadja Zeltner^1,2,3

¹Center for Molecular Medicine,University of Georgia, ²Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, ³Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

概要

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

Explore More Videos

Human Pluripotent Stem Cells Sympathetic Neurons Feeder-free Chemically Defined Efficient Differentiation Electrical Activity Disease Modeling Regenerative Medicine Drug Screening Co-culture System

Read Article

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

概要

Automatically generated

Explore More Videos