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Exon Skiping en Miotubos Directamente Reprogramados obtenidos de Células Derivadas de orina humana
JoVE Journal
Genetics
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JoVE Journal Genetics
Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells
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Exon Skiping en Miotubos Directamente Reprogramados obtenidos de Células Derivadas de orina humana

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6,742 Views

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06:20 min

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May 07, 2020

DOI:

10.3791/60840-v

DOI:
10.3791/60840-v

•

06:20 min
May 07, 2020

•

2 Views
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1Department of Molecular Therapy, National Institute of Neuroscience,National Center of Neurology and Psychiatry

Summary

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En este artículo, describimos un protocolo detallado para el modelado eficiente del músculo de la distrofia muscular de Duchenne usando células derivadas de orina convertidas MYOD1para evaluar la restauración de los niveles de ARNm de distrofina y proteína según el exon.

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Takizawa, H., Sato, M., Aoki, Y. Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells. J. Vis. Exp. (159), e60840, doi:10.3791/60840 (2020).

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