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Visualización y análisis de arterias de arco faríngeo utilizando inmunohistoquímica de montaje completo y reconstrucción 3D
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Developmental Biology
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JoVE Journal Developmental Biology
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
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Visualización y análisis de arterias de arco faríngeo utilizando inmunohistoquímica de montaje completo y reconstrucción 3D

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9,300 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:
10.3791/60797-v

•

10:02 min
March 31, 2020

•

1 Views
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1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Summary

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Aquí, describimos un protocolo para visualizar y analizar las arterias del arco faríngeo 3, 4 y 6 de embriones de ratón utilizando inmunofluorescencia de montaje completo, limpieza de tejidos, microscopía confocal y reconstrucción 3D.

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Cite this Article

Ramirez, A., Astrof, S. Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction. J. Vis. Exp. (157), e60797, doi:10.3791/60797 (2020).

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