ジャーナル
/
発生生物学
/
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

DOI:
10.3791/60797-v

•

10:02 min

•

March 31, 2020

•

,
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

章

  • 00:04Introduction
  • 00:55Embryo Staining and Embedding
  • 03:03Methanol Dehydration and Tissue Clearing
  • 04:04Embryo Mounting
  • 04:40Third Pharyngeal Arch (PA) Surfacing
  • 05:57Surfaced Structure Masking
  • 07:01Endothelial Cell Quantification
  • 08:19Results: Representative PA and PAA Surfacing and EC Quantification
  • 09:28Conclusion

概要

自動翻訳

自動翻訳

Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction.

タグ

VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Ex Vivo Culture of Pharyngeal Arches to Study Heart and Muscle Progenitors and Their Niche

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Read Article