Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

AnnJosette Ramirez; Sophie Astrof

doi:10.3791/60797

ジャーナル

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発生生物学

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Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

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発生生物学

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JoVE Journal 発生生物学

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

9,300 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:

10.3791/60797-v

•

10:02 min

March 31, 2020

•

1 Views

AnnJosette Ramirez^1,2, Sophie Astrof¹

¹Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, ²Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

概要

Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction.

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Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

概要

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