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Lokalisatie en kwantificering van Begomovirussen in Whitefly Weefsels door immunofluorescentie en kwantitatieve PCR
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Immunology and Infection
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JoVE Journal Immunology and Infection
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Lokalisatie en kwantificering van Begomovirussen in Whitefly Weefsels door immunofluorescentie en kwantitatieve PCR

Nederlands

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5,403 Views

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06:25 min

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February 08, 2020

DOI:

10.3791/60731-v

DOI:
10.3791/60731-v

•

06:25 min
February 08, 2020

•

3 Views
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1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Summary

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We beschrijven een immunofluorescentie en kwantitatieve PCR-methode voor de lokalisatie en kwantificering van begomovirussen in insectenweefsels. Het immunofluorescentieprotocol kan worden gebruikt om virale en vectoreiwitten samen te lokaliseren. Het kwantitatieve PCR-protocol kan worden uitgebreid om virussen in hele wittevlieglichamen en met virussen geïnfecteerde planten te kwantificeren.

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Ban, F., Yin, T., Guo, Q., Pan, L., Liu, Y., Wang, X. Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J. Vis. Exp. (156), e60731, doi:10.3791/60731 (2020).

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