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Immunology and Infection
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澄清和成像念珠菌生物膜
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Clarifying and Imaging Candida albicans Biofilms
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澄清和成像念珠菌生物膜

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10,827 Views

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11:09 min

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March 06, 2020

DOI:

10.3791/60718-v

DOI:
10.3791/60718-v

•

11:09 min
March 06, 2020

•

58 Views
, , , , ,
1Department of Biological Sciences,Carnegie Mellon University, 2Department of Pharmacology,Second Military Medical University

Summary

Automatically generated

为了查看和量化念珠菌生物膜的内部特征，我们制备了固定完整的标本，通过折射率匹配进行澄清。然后，利用光学切片显微镜通过生物膜全厚度获取三维图像数据。

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Cite this Article

Lanni, F., Lagree, K., Huang, M. Y., Yan, L., Woolford, C. A., Mitchell, A. P. Clarifying and Imaging Candida albicans Biofilms. J. Vis. Exp. (157), e60718, doi:10.3791/60718 (2020).

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