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Journal / Immunology and Infection / Clarifying and Imaging Candida albicans Biofilms
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Clarifying and Imaging Candida albicans Biofilms
 

Clarifying and Imaging Candida albicans Biofilms

Article DOI: 10.3791/60718-v • 11:09 min • March 6th, 2020
March 6th, 2020 •
1, 1, 1, 2, 1, 1
1Department of Biological Sciences, Carnegie Mellon University, 2Department of Pharmacology, Second Military Medical University

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Summary
Automatic Translation

To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.

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Keywords: Candida Albicans Biofilm Clarification Optical Sectioning Confocal Microscopy Fixation Staining Transparency 3D Imaging
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