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Clarifying and Imaging Candida albicans Biofilms
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Clarifying and Imaging Candida albicans Biofilms

Clarifying and Imaging Candida albicans Biofilms

DOI:
10.3791/60718-v

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11:09 min

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March 06, 2020

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, , , , ,
1Department of Biological Sciences,Carnegie Mellon University, 2Department of Pharmacology,Second Military Medical University

Chapters

  • 00:04Introduction
  • 00:48Growth of Biofilm Cultures
  • 01:34Specimen Processing for Imaging
  • 02:39Clarification Protocol: Biofilms on Impermeant Substrata
  • 04:30Imaging Setup for Confocal Microscopy
  • 05:41Fast Image Processing and Display for Large Confocal Image Stacks
  • 08:21Results: Optimum Index Matching and Deep Imaging of Mutant and Wild Type C. albicans Biofilms
  • 09:49Conclusion

Summary

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To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.

Tags

Candida AlbicansBiofilmClarificationOptical SectioningConfocal MicroscopyFixationStainingTransparency3D Imaging

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