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Clarifying and Imaging Candida albicans Biofilms
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March 06, 2020
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To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm.
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Lanni, F., Lagree, K., Huang, M. Y., Yan, L., Woolford, C. A., Mitchell, A. P. Clarifying and Imaging Candida albicans Biofilms. J. Vis. Exp. (157), e60718, doi:10.3791/60718 (2020).
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