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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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Genetics
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JoVE Journal Genetics
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

3,892 Views

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08:20 min

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September 02, 2021

DOI:

10.3791/60685-v

DOI:
10.3791/60685-v

•

08:20 min
September 02, 2021

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4 Views
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1Cold Spring Harbor Laboratory, 2Faculty of Pharmacy,University of Ljubljana

Tags

CRISPR-Cas9Gene EditingConditional Cas9 StabilizationShield-1Essential GenesTumor Survival GenesDD-Cas9In VivoIn VitroBlood-brain BarrierHEK293T CellsTransfectionVirus SupernatantVirus Titer

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Cite this Article

Šafarič Tepeš, P., Sordella, R. A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization. J. Vis. Exp. (175), e60685, doi:10.3791/60685 (2021).

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