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使用"彩虹"和"延时共聚焦成像"可视化活斑马鱼中发育中的脑
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
DOI:

07:28 min

March 23, 2020

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Chapters

  • 00:05Introduction
  • 00:53Microinjection and Heat Shock of Zebrafish Embryos
  • 02:06Screening and Mounting Embryos
  • 03:59Time-lapse Confocal Imaging of the Developing Zebrafish Hindbrain
  • 05:17Results: Brainbow Labels Clonally Related Clusters of Dividing Cells in the Developing Zebrafish Hindbrain
  • 06:55Conclusion

Summary

Automatic Translation

体内成像是一种强大的工具,可用于研究神经系统发育背后的细胞机制。在这里,我们描述了一种技术,使用延时共聚焦显微镜,以可视化大量的多色彩虹标记细胞在发展中的斑马鱼神经系统实时。

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