JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
使用"彩虹"和"延时共聚焦成像"可视化活斑马鱼中发育中的脑
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
Please note that all translations are automatically generated.
Click here for the English version.
使用"彩虹"和"延时共聚焦成像"可视化活斑马鱼中发育中的脑
DOI:
10.3791/60593-v
•
07:28 min
•
March 23, 2020
•
Zoe T. Cook
,
Nicole L. Brockway
,
Tamily A. Weissman
1
Biology Department
,
Lewis & Clark College
Chapters
00:05
Introduction
00:53
Microinjection and Heat Shock of Zebrafish Embryos
02:06
Screening and Mounting Embryos
03:59
Time-lapse Confocal Imaging of the Developing Zebrafish Hindbrain
05:17
Results: Brainbow Labels Clonally Related Clusters of Dividing Cells in the Developing Zebrafish Hindbrain
06:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
体内成像是一种强大的工具,可用于研究神经系统发育背后的细胞机制。在这里,我们描述了一种技术,使用延时共聚焦显微镜,以可视化大量的多色彩虹标记细胞在发展中的斑马鱼神经系统实时。
Tags
Brain Development
Neural Progenitor Cells
Clonally Related Cells
Zebrafish
Brainbow
Time-lapse Confocal Imaging
Fluorescence Microscopy
PTU
Neural Development
Embryo
Heat Shock
CFP
YFP
Brainbow Recombination
Confocal Time-lapse Imaging
Imaging Chamber
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测量生活中的斑马鱼胚胎蛋白质稳定性使用荧光衰减光转化后(FDAP)
捕获组织修复的斑马鱼幼虫与时间推移明视立体显微镜
在活斑马鱼胚胎成像的亚细胞结构
在GFP-转基因斑马鱼使用Photoconvertible PSmOrange系统追踪单元
分析<em>在体内</em>细胞迁移使用细胞移植和时间推移成像在斑马鱼胚胎
配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析
可视化Interrenal类固醇合成组织及其血管微环境斑马鱼
住发展拟南芥花的共焦成像
诱导生活青蛙和斑马鱼胚胎缺氧
多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
Read Article