Journal
/
Biology
/
A High Output Method to Isolate Cerebral Pericytes from Mouse
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A High Output Method to Isolate Cerebral Pericytes from Mouse

A High Output Method to Isolate Cerebral Pericytes from Mouse

English

Automatically Generated

7,928 Views

•

06:49 min

•

January 14, 2020

DOI:

10.3791/60588-v

DOI:
10.3791/60588-v

•

06:49 min
January 14, 2020

•

8 Views
, , , , ,
1Laboratory of the Blood Brain Barrier,University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Summary

Automatically generated

We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.

Explore More Videos

Cerebral PericytesPericyte IsolationMouse BrainIn Vitro StudiesCell CultureCell YieldHigh PurityHomogenizationVascular FractionBovine Serum Albumin DextranCell SuspensionFiltration

Read Article

Cite this Article

Mehra, A., Dehouck, L., Vandenhaute, E., Fatar, M., Fenart, L., Gosselet, F. A High Output Method to Isolate Cerebral Pericytes from Mouse. J. Vis. Exp. (155), e60588, doi:10.3791/60588 (2020).

Download .ris file

Copy

Share Video

.

Copy