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A High Output Method to Isolate Cerebral Pericytes from Mouse
Chapters
- 00:04Title
- 00:44Brain Tissue Recovery and Meninges Removal
- 01:31Homogenization
- 02:18Vascular Fraction Isolation
- 04:32Cerebral Pericyte Proliferation
- 05:25Results: Representative Cell Morphology, Marker Expression, and Culture Purity
- 06:25Conclusion
We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.
