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A High Output Method to Isolate Cerebral Pericytes from Mouse
JoVE Journal
Biologia
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JoVE Journal Biologia
A High Output Method to Isolate Cerebral Pericytes from Mouse

A High Output Method to Isolate Cerebral Pericytes from Mouse

7,928 Views

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06:49 min

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January 14, 2020

DOI:

10.3791/60588-v

DOI:
10.3791/60588-v

•

06:49 min
January 14, 2020

•

8 Views
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1Laboratory of the Blood Brain Barrier,University of Artois, 2Department of Neurology, Universitätsmedizin Mannheim,Heidelberg University

Summary

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We present a protocol for the extraction of murine cerebral pericytes. Based on an antibiotic-free enrichment oriented pericyte extraction, this protocol is a valuable tool for in vitro studies providing high purity and high yield, thus decreasing the number of experimental animals used.

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Cite this Article

Mehra, A., Dehouck, L., Vandenhaute, E., Fatar, M., Fenart, L., Gosselet, F. A High Output Method to Isolate Cerebral Pericytes from Mouse. J. Vis. Exp. (155), e60588, doi:10.3791/60588 (2020).

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