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In vitro stimulatie en visualisatie van extracellulaire val afgifte in gedifferentieerde humane monocyte-afgeleide macrofagen
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
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In vitro stimulatie en visualisatie van extracellulaire val afgifte in gedifferentieerde humane monocyte-afgeleide macrofagen

Nederlands

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9,299 Views

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08:08 min

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November 01, 2019

DOI:

10.3791/60541-v

DOI:
10.3791/60541-v

•

08:08 min
November 01, 2019

•

14 Views
, , ,
1Heart Research Institute, 2Sydney Medical School,University of Sydney, 3Department of Biomedical Sciences,University of Copenhagen

Summary

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Hier is een protocol voor het opsporen van macrofaag extracellulaire trap (met) productie in levende celcultuur met behulp van microscopie en fluorescentie kleuring. Dit protocol kan verder worden uitgebreid om specifieke MET eiwit markers te onderzoeken door immunofluorescentie kleuring.

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Cite this Article

Zhang, Y., Rayner, B. S., Jensen, M., Hawkins, C. L. In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages. J. Vis. Exp. (153), e60541, doi:10.3791/60541 (2019).

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