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In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

9,299 Views

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08:08 min

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November 01, 2019

DOI:

10.3791/60541-v

DOI:
10.3791/60541-v

•

08:08 min
November 01, 2019

•

14 Views
, , ,
1Heart Research Institute, 2Sydney Medical School,University of Sydney, 3Department of Biomedical Sciences,University of Copenhagen

Summary

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Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

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Cite this Article

Zhang, Y., Rayner, B. S., Jensen, M., Hawkins, C. L. In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages. J. Vis. Exp. (153), e60541, doi:10.3791/60541 (2019).

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