Journal
/
Inmunología y contagio
/
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

9,299 Views

•

08:08 min

•

November 01, 2019

DOI:

10.3791/60541-v

DOI:
10.3791/60541-v

•

08:08 min
November 01, 2019

•

14 Views
, , ,
1Heart Research Institute, 2Sydney Medical School,University of Sydney, 3Department of Biomedical Sciences,University of Copenhagen

Summary

Automatically generated

Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining.

Explore More Videos

Extracellular TrapMacrophagesNeutrophilsImmune CellsInnate ImmunityInflammatory PathologiesIn Vitro ModelPrimary Human CellsMonocyte-derived MacrophagesM1 PrimingM2 PrimingHypochlorous AcidPMATNF-alphaIL-8 Stimulation

Read Article

Cite this Article

Zhang, Y., Rayner, B. S., Jensen, M., Hawkins, C. L. In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages. J. Vis. Exp. (153), e60541, doi:10.3791/60541 (2019).

Download .ris file

Copy

Share Video

.

Copy