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Pseudomonas aeruginosa Küçük Koloni Varyant Kültürü ve Aljinat Quantitation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
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Pseudomonas aeruginosa Küçük Koloni Varyant Kültürü ve Aljinat Quantitation

Türkçe

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8,180 Views

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09:06 min

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February 22, 2020

DOI:

10.3791/60466-v

DOI:
10.3791/60466-v

•

09:06 min
February 22, 2020

•

5 Views
, ,
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Summary

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Burada, pseudomonas aeruginosaküçük koloni varyantı kültüre bir büyüme durumu açıklar. Ayrıca p. aeruginosa tarafından üretilen eksopolisakkarit aljinatının saptanması ve nicelliği için geleneksel üronik asit karbazol testi ve aljinatspesifik monoklonal antikor (mAb) bazlı ELISA kullanılarak iki ayrı yöntem tanımlanmıştır.

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Cite this Article

Al Ahmar, R., Kirby, B. D., Yu, H. D. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (156), e60466, doi:10.3791/60466 (2020).

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