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Culture de la petite colonie Variante de Pseudomonas aeruginosa et Quantitation de son Alginate
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Immunology and Infection
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JoVE Journal Immunology and Infection
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
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Culture de la petite colonie Variante de Pseudomonas aeruginosa et Quantitation de son Alginate

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8,180 Views

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09:06 min

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February 22, 2020

DOI:

10.3791/60466-v

DOI:
10.3791/60466-v

•

09:06 min
February 22, 2020

•

5 Views
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1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Summary

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Ici, nous décrivons une condition de croissance à la culture de la variante petite colonie de Pseudomonas aeruginosa. Nous décrivons également deux méthodes séparées pour la détection et la quantitation de l’alginate d’exopolysaccharide produit par P. aeruginosa utilisant un test traditionnel de carbazole d’acide uronique et un anticorps monoclonal alginate-spécifique (mAb) basé ELISA.

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Al Ahmar, R., Kirby, B. D., Yu, H. D. Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate. J. Vis. Exp. (156), e60466, doi:10.3791/60466 (2020).

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