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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Chapters
- 00:04Introduction
- 00:49SCV Growth Conditions and Physiological Activation of the Salvage Pathway
- 01:47Uronic Acid Carbazole Assay
- 04:36ELISA for Alginate Quantitation
- 06:34Results: Uronic Acid Carbazole Assay, Anti-Alginate mAb-Based ELISA, and Direct Patient Sample Testing
- 08:15Conclusion
Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.
