JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
一个集成拉曼光谱和质谱平台,用于研究单细胞药物接受、代谢和效果
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Please note that all translations are automatically generated.
Click here for the English version.
一个集成拉曼光谱和质谱平台,用于研究单细胞药物接受、代谢和效果
DOI:
10.3791/60449-v
•
07:37 min
•
January 09, 2020
•
Ahmed Ali
2
,
Yasmine Abouleila
2
,
Arno Germond
1
Biodynamics Research Center (BDR), RIKEN
,
2
Research Center
,
Misr International University
Chapters
00:04
Title
01:06
Cell Culture
01:44
Drug Treatment
02:33
Raman Spectral Imaging and Spectral Processing
03:29
Single-cell Sampling Set-up and Procedures, and Mass Spectrometry Measurements
05:34
Results: Drug-treated and Untreated Cells
06:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议提供了一个集成的拉曼光谱-质谱(MS)平台,能够实现单细胞分辨率。拉曼光谱可用于研究细胞对药物的反应,而MS可用于药物获取和代谢的靶向和定量分析。
Tags
Raman Spectroscopy
Mass Spectrometry
Single-cell Analysis
Drug Uptake
Metabolism
Cellular Heterogeneity
Cancer Resistance
Metabolomics
Spectral Imaging
Micromanipulator
Cell Culture
Tamoxifen
DMSO
Spectrophotometer Calibration
Micro-chamber
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
鞘毛细管电泳 - 质谱联用生物样品的代谢谱
制造的UV-Vis和拉曼光谱免疫平台
时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物
脂滴隔离定量质谱分析
通过肽浓缩和质谱检测蛋白质泛化位点检测
血管紧张蛋白II图解的硝酸分析与鉴定
使用自动工作站进行质谱测量的等离子体样品制备
探头电喷电镀质谱的样品制备
通过反相液相-质谱法对无细胞蛋白合成代谢的绝对定量
Read Article