Journal
/
Bioquímica
/
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

8,937 Views

•

07:37 min

•

January 09, 2020

DOI:

10.3791/60449-v

DOI:
10.3791/60449-v

•

07:37 min
January 09, 2020

•

8 Views
, ,
1Biodynamics Research Center (BDR), RIKEN, 2Research Center,Misr International University

Summary

Automatically generated

This protocol presents an integrated Raman spectroscopy-mass spectrometry (MS) platform that is capable of achieving single-cell resolution. Raman spectroscopy can be used to study cellular response to drugs, while MS can be used for targeted and quantitative analysis of drug uptake and metabolism.

Explore More Videos

Raman SpectroscopyMass SpectrometrySingle-cell AnalysisDrug UptakeMetabolismCellular HeterogeneityCancer ResistanceMetabolomicsSpectral ImagingMicromanipulatorCell CultureTamoxifenDMSOSpectrophotometer CalibrationMicro-chamber

Read Article

Cite this Article

Ali, A., Abouleila, Y., Germond, A. An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects. J. Vis. Exp. (155), e60449, doi:10.3791/60449 (2020).

Download .ris file

Copy

Share Video

.

Copy