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Developmental Biology

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多细胞人类原发性子宫内膜器官的生成

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Developmental Biology

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JoVE Journal Developmental Biology

Generation of Multicellular Human Primary Endometrial Organoids

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多细胞人类原发性子宫内膜器官的生成

DOI:

10.3791/60384-v

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09:20 min

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October 04, 2019

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Alina R. Murphy*¹, Teerawat Wiwatpanit*², Zhenxiao Lu, Batzaya Davaadelger, J. Julie Kim

¹Department of Obstetrics and Gynecology,Northwestern University, ²National Center for Genetic Engineering and Biotechnology (BIOTEC),National Science and Technology Development Agency (NSTDA)

Chapters

00:04Title
01:39Preparation of Agarose Molds and Generation of Endometrial Organoids
04:58Harvesting Endometrial Organoids for Experiments
06:40Results: Analysis of Generated Multicellular Human Primary Endometrial Organoids
08:10Conclusion

Summary

Automatic Translation

提出了一种产生人类原发子宫内膜器官的方案，该细胞由上皮细胞和基质细胞组成，并保留原生子宫内膜组织的特性。该协议描述了从子宫组织采集到子宫内膜器官组织处理的方法。

Tags

Keywords: Endometrium Organoids 3D Structure Epithelial Cells Stromal Cells Hormone Imbalance Cancer Paracrine Actions Cell Isolation Enzyme Solution Cell Strainer

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