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Developmental Biology
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マウスマンモスフィア培養における自己再生の定量
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Quantification of Self-renewal in Murine Mammosphere Cultures
Please note that all translations are automatically generated.
Click here for the English version.
マウスマンモスフィア培養における自己再生の定量
DOI:
10.3791/60256-v
•
07:40 min
•
November 26, 2019
•
Thalia Vlachou
,
Xieraili Aobuli
,
Errico D’Elia
,
Angela Santoro
,
Maria Cristina Moroni
,
Pier Giuseppe Pelicci
2
1
Department of Experimental Oncology, IEO
,
European Institute of Oncology IRCCS
,
2
Department of Oncology and Hemato-Oncology
,
University of Milan
Chapters
00:04
Title
01:09
Murine Mammary Gland Collection and Digestion
01:52
Primary Murine Mammary Epithelial Cell (MEC) Isolation
03:15
Serial Mammosphere Re-Plating
04:12
Sphere Enumeration by Digital Image Analysis (DIA)
05:45
Cumulative Growth Curve Calculation
06:38
Results: Representative Sphere and Cell Growth Numbers and Curves
07:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、インビトロマンモスフィア自己再生定量アッセイの実施と解釈について説明する。
Tags
Mammosphere Culture
Self-renewal
Breast Stem Cells
Quantification
Cell Culture
Cell Isolation
Cell Counting
Cell Lysis
Ultra-low Adhesion
Primary Mammosphere Formation
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