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Quantification of Self-renewal in Murine Mammosphere Cultures
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Quantification of Self-renewal in Murine Mammosphere Cultures

Quantification of Self-renewal in Murine Mammosphere Cultures

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07:40 min

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November 26, 2019

DOI:

10.3791/60256-v

DOI:
10.3791/60256-v

•

07:40 min
November 26, 2019

•

2 Views
, , , , ,
1Department of Experimental Oncology, IEO,European Institute of Oncology IRCCS, 2Department of Oncology and Hemato-Oncology,University of Milan

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Here, we describe the implementation and interpretation of the results of an in vitro mammosphere self-renewal quantitative assay.

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Mammosphere CultureSelf-renewalBreast Stem CellsQuantificationCell CultureCell IsolationCell CountingCell LysisUltra-low AdhesionPrimary Mammosphere Formation

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Vlachou, T., Aobuli, X., D'Elia, E., Santoro, A., Moroni, M. C., Pelicci, P. G. Quantification of Self-renewal in Murine Mammosphere Cultures. J. Vis. Exp. (153), e60256, doi:10.3791/60256 (2019).

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