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Quantification of Self-renewal in Murine Mammosphere Cultures
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Quantification of Self-renewal in Murine Mammosphere Cultures

Quantification of Self-renewal in Murine Mammosphere Cultures

DOI:
10.3791/60256-v

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07:40 min

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November 26, 2019

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, , , , ,
1Department of Experimental Oncology, IEO,European Institute of Oncology IRCCS, 2Department of Oncology and Hemato-Oncology,University of Milan

Capítulos

  • 00:04Título
  • 01:09Murine Mammary Gland Collection and Digestion
  • 01:52Primary Murine Mammary Epithelial Cell (MEC) Isolation
  • 03:15Serial Mammosphere Re-Plating
  • 04:12Sphere Enumeration by Digital Image Analysis (DIA)
  • 05:45Cumulative Growth Curve Calculation
  • 06:38Results: Representative Sphere and Cell Growth Numbers and Curves
  • 07:05Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we describe the implementation and interpretation of the results of an in vitro mammosphere self-renewal quantitative assay.

Tags

Mammosphere CultureSelf-renewalBreast Stem CellsQuantificationCell CultureCell IsolationCell CountingCell LysisUltra-low AdhesionPrimary Mammosphere Formation

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