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Analysis of Hematopoietic Stem Progenitor Cell Metabolism
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Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

English

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6,553 Views

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12:20 min

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November 09, 2019

DOI:

10.3791/60234-v

DOI:
10.3791/60234-v

•

12:20 min
November 09, 2019

•

19 Views
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1Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

Summary

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Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

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Cite this Article

Scapin, G., Goulard, M. C., Dharampuriya, P. R., Cillis, J. L., Shah, D. I. Analysis of Hematopoietic Stem Progenitor Cell Metabolism. J. Vis. Exp. (153), e60234, doi:10.3791/60234 (2019).

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