Dergi
/
Gelişim Biyolojisi
/
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
JoVE Journal
Gelişim Biyolojisi
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Gelişim Biyolojisi
Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

6,551 Views

•

12:20 min

•

November 09, 2019

DOI:

10.3791/60234-v

DOI:
10.3791/60234-v

•

12:20 min
November 09, 2019

•

19 Views
, , , ,
1Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

Özet

Automatically generated

Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

Explore More Videos

Hematopoietic Stem CellsHematopoietic Progenitor CellsMetabolic RegulationMitochondrial RespirationGlycolysisExtracellular Flux AnalysisMurine Bone MarrowDensity GradientMononucleated Cells

Read Article

Cite this Article

Scapin, G., Goulard, M. C., Dharampuriya, P. R., Cillis, J. L., Shah, D. I. Analysis of Hematopoietic Stem Progenitor Cell Metabolism. J. Vis. Exp. (153), e60234, doi:10.3791/60234 (2019).

Download .ris file

Copy

Share Video

.

Copy