Journal
/
Biologia do Desenvolvimento
/
Analysis of Hematopoietic Stem Progenitor Cell Metabolism
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

6,553 Views

•

12:20 min

•

November 09, 2019

DOI:

10.3791/60234-v

DOI:
10.3791/60234-v

•

12:20 min
November 09, 2019

•

19 Views
, , , ,
1Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

Summary

Automatically generated

Hematopoietic stem progenitor cells (HSPCs) transition from a quiescent state to a differentiation state due to their metabolic plasticity during blood formation. Here, we present an optimized method for measuring mitochondrial respiration and glycolysis of HSPCs.

Explore More Videos

Hematopoietic Stem CellsHematopoietic Progenitor CellsMetabolic RegulationMitochondrial RespirationGlycolysisExtracellular Flux AnalysisMurine Bone MarrowDensity GradientMononucleated Cells

Read Article

Cite this Article

Scapin, G., Goulard, M. C., Dharampuriya, P. R., Cillis, J. L., Shah, D. I. Analysis of Hematopoietic Stem Progenitor Cell Metabolism. J. Vis. Exp. (153), e60234, doi:10.3791/60234 (2019).

Download .ris file

Copy

Share Video

.

Copy