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通过胚胎移植，生成具有突变倍体肢体的奇美体轴球
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Genetics
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JoVE Journal Genetics
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
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通过胚胎移植，生成具有突变倍体肢体的奇美体轴球

DOI:
10.3791/60156-v

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07:17 min

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January 29, 2020

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, ,
1Department of Molecular, Cellular, and Developmental Biology,Yale University

Chapters

  • 00:05Title
  • 00:48Female Gamete Donor Preparation
  • 01:36Male Gamete Collection and Preparation
  • 02:51Female Gamete Collection
  • 03:48Haploid-Diploid Chimera Generation
  • 05:07Results: Representative Haploid and Diploid Limb Development
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目标是使用胚胎组织移植技术，从Cas9诱变的供体组织中提取单倍体前肢的嵌合体轴片。

Tags

Chimeric AxolotlsHaploid LimbsEmbryonic GraftingGenetic ScreeningRegenerationAmphibianIn Vitro FertilizationGamete DonorChorionic GonadotropinSpermic UrineSperm IrradiationCrosslinker

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