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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer par CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
JoVE Journal
Genetics
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JoVE Journal Genetics
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer par CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

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7,098 Views

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09:16 min

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September 01, 2019

DOI:

10.3791/60130-v

DOI:
10.3791/60130-v

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09:16 min
September 01, 2019

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6 Views
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1Blood Cancer Cytogenetics and Genomics Laboratory, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital,The Chinese University of Hong Kong, 2State Key Laboratory in Oncology in South China,The Chinese University of Hong Kong

Summary

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La livraison directe des complexes préassemblés de ribonucleoprotein d'ARN de Cas9/guide est un moyen rapide et efficace pour l'édition de génome dans les cellules hématopoïétiques. Ici, nous utilisons cette approche pour supprimer un silencieux intronique RUNX1 et examiner les réponses transcriptionnelles dans les cellules leucémiques OCI-AML3.

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Keyword Extraction:RUNX1Intronic SilencerCRISPR/Cas9 RibonucleoproteinAcute Myeloid LeukemiaCis-regulatory ElementsGene ExpressionTranscriptional RoleHematopoietic CellsElectroporationCas9 NucleaseGRNARNP ComplexFragment Analysis

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Cite this Article

Cheng, C., Wong, T. H., Yung, Y., Chan, N. C., Ng, M. H. Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells. J. Vis. Exp. (151), e60130, doi:10.3791/60130 (2019).

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