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Genetics

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JoVE Journal Genetics
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
 

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Article DOI: 10.3791/60130-v • 09:16 min • September 1st, 2019
September 1st, 2019 •
1, 1, 1, 1, 1,2
1Blood Cancer Cytogenetics and Genomics Laboratory, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, 2State Key Laboratory in Oncology in South China, The Chinese University of Hong Kong

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Automatic Translation

Direct delivery of preassembled Cas9/guide RNA ribonucleoprotein complexes is a fast and efficient means for genome editing in hematopoietic cells. Here, we utilize this approach to delete a RUNX1 intronic silencer and examine the transcriptional responses in OCI-AML3 leukemic cells.

Tags

Transcriptional Role RUNX1 Intronic Silencer CRISPR/Cas9 Ribonucleoprotein Acute Myeloid Leukemia Cells Cis-regulatory Elements Gene Expression Heterologous Reporter Genes CRISPR Leukemia Cell Line Gene Regulatory Functions Endogenous Gene Context Hematopoietic Cells Electroporation Plasmid-based Methods Editing Efficiency Cell Viability Off-target Effects Fragment Analysis Mutant Clones CRISPR RNAs PAM Recognition
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