Investigation of the Transcriptional Role of a <em>RUNX1</em> Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Chi-Keung Cheng; Terry  H.Y. Wong; Yuk-Lin Yung; Nelson  C.N. Chan; Margaret  H.L. Ng

doi:10.3791/60130

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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

JoVE Journal
遺伝学

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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

7,095 Views

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09:16 min

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September 01, 2019

DOI:

10.3791/60130-v

DOI:

10.3791/60130-v

•

09:16 min

September 01, 2019

•

6 Views

Chi-Keung Cheng¹, Terry H.Y. Wong¹, Yuk-Lin Yung¹, Nelson C.N. Chan¹, Margaret H.L. Ng^1,2

¹Blood Cancer Cytogenetics and Genomics Laboratory, Department of Anatomical and Cellular Pathology, Prince of Wales Hospital,The Chinese University of Hong Kong, ²State Key Laboratory in Oncology in South China,The Chinese University of Hong Kong

概要

Direct delivery of preassembled Cas9/guide RNA ribonucleoprotein complexes is a fast and efficient means for genome editing in hematopoietic cells. Here, we utilize this approach to delete a RUNX1 intronic silencer and examine the transcriptional responses in OCI-AML3 leukemic cells.

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Keyword Extraction:RUNX1 Intronic Silencer CRISPR/Cas9 Ribonucleoprotein Acute Myeloid Leukemia Cis-regulatory Elements Gene Expression Transcriptional Role Hematopoietic Cells Electroporation Cas9 Nuclease GRNA RNP Complex Fragment Analysis

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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

概要

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