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Reprogramación hemogénica de fibroblastos humanos por expresión forzada de factores de transcripción
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November 04, 2019
DOI:
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, 2Wallenberg Center for Molecular,Lund University, 3Center for Neuroscience and Cell Biology,University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai
Summary
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Este protocolo demuestra la inducción de un programa hemogénico en fibroblastos dérmicos humanos mediante la expresión forzada de los factores de transcripción GATA2, GFI1B y FOS para generar células hematopoyéticas madre y progenitoras.
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Silvério-Alves, R., Gomes, A. M., Kurochkin, I., Moore, K. A., Pereira, C. Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors. J. Vis. Exp. (153), e60112, doi:10.3791/60112 (2019).
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