/
/
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
This content is Free Access.
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
5,696 Views
•
11:42 min
•
November 04, 2019
DOI:
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, 2Wallenberg Center for Molecular,Lund University, 3Center for Neuroscience and Cell Biology,University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai
Summary
Automatically generated
This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.
Read Article
Cite this Article
Silvério-Alves, R., Gomes, A. M., Kurochkin, I., Moore, K. A., Pereira, C. Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors. J. Vis. Exp. (153), e60112, doi:10.3791/60112 (2019).
Copy