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Developmental Biology

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転写因子の強制発現によるヒト線維芽細胞のヘモジェニックリプログラミング

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

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転写因子の強制発現によるヒト線維芽細胞のヘモジェニックリプログラミング

5,689 Views

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11:42 min

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November 04, 2019

DOI:

10.3791/60112-v

DOI:

10.3791/60112-v

•

11:42 min

November 04, 2019

•

4 Views

Rita Silvério-Alves^1,2,3, Andreia M. Gomes³, Ilia Kurochkin⁴, Kateri A. Moore^5,6, Carlos-Filipe Pereira^1,2,3

¹Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, ²Wallenberg Center for Molecular,Lund University, ³Center for Neuroscience and Cell Biology,University of Coimbra, ⁴Skolkovo Institute of Science and Technology, ⁵Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, ⁶Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Summary

このプロトコルは、血球性幹および前駆細胞を生成する転写因子GATA2、GFI1BおよびFOSの強制発現によるヒト皮膚線維芽細胞における血球形成プログラムの誘導を示す。

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