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Trimerik Epitop Sunumu Gerektiren Aşılar için E. coli-expressed Self-Assembling Protein Nano tanecikleri Üretimi
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
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Trimerik Epitop Sunumu Gerektiren Aşılar için E. coli-expressed Self-Assembling Protein Nano tanecikleri Üretimi

Türkçe

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7,209 Views

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00:10 min

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August 21, 2019

DOI:

10.3791/60103-v

DOI:
10.3791/60103-v

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00:10 min
August 21, 2019

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3 Views
, , ,
1U.S. Military HIV Research Program,Walter Reed Army Institute of Research, 2Henry M. Jackson Foundation for the Advancement of Military Medicine, 3Alpha-O Peptides AG

Summary

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Aşı geliştirmede kullanılmak üzere kendi kendine biraraya gelen protein nano taneciklerinin (SAPN' ler) saflaştırılmasını, yeniden katlanması ve karakterizasyonu nun tanımlanmasını açıklayan ayrıntılı bir yöntem burada verilmiştir.

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Cite this Article

Karch, C. P., Burkhard, P., Matyas, G. R., Beck, Z. Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation. J. Vis. Exp. (150), e60103, doi:10.3791/60103 (2019).

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