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トリマーエピトーププレゼンテーションを必要とするワクチンに対する大腸菌の自己組織化タンパク質ナノ粒子の産生
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
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トリマーエピトーププレゼンテーションを必要とするワクチンに対する大腸菌の自己組織化タンパク質ナノ粒子の産生

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7,209 Views

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00:10 min

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August 21, 2019

DOI:

10.3791/60103-v

DOI:
10.3791/60103-v

•

00:10 min
August 21, 2019

•

3 Views
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1U.S. Military HIV Research Program,Walter Reed Army Institute of Research, 2Henry M. Jackson Foundation for the Advancement of Military Medicine, 3Alpha-O Peptides AG

Summary

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ここでは、ワクチン開発に使用する自己組織化タンパク質ナノ粒子(SAPN)の精製、再折りたたみ、および特性評価について詳しく説明します。

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Cite this Article

Karch, C. P., Burkhard, P., Matyas, G. R., Beck, Z. Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation. J. Vis. Exp. (150), e60103, doi:10.3791/60103 (2019).

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