Production of <em>E. coli</em>-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Christopher  P. Karch; Peter Burkhard; Gary  R. Matyas; Zoltan Beck

doi:10.3791/60103

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Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

7,209 Views

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00:10 min

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August 21, 2019

DOI:

10.3791/60103-v

DOI:

10.3791/60103-v

•

00:10 min

August 21, 2019

•

3 Views

Christopher P. Karch^1,2, Peter Burkhard³, Gary R. Matyas¹, Zoltan Beck^1,2

¹U.S. Military HIV Research Program,Walter Reed Army Institute of Research, ²Henry M. Jackson Foundation for the Advancement of Military Medicine, ³Alpha-O Peptides AG

Summary

A detailed method is provided here describing the purification, refolding, and characterization of self-assembling protein nanoparticles (SAPNs) for use in vaccine development.

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E. Coli Self-assembling Protein Nanoparticles SAPN Vaccines Trimeric Epitope Presentation Protein Purification Protein Refolding Protein Characterization Six-helix Bundle FPLC Affinity Chromatography HisTrap HP Column Protein Expression Large-scale Production Clinical Trials

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Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Summary

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