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Sistema de ensayo de angiogénesis tridimensional utilizando esferoides de cocultura formados por células formadoras de colonias endoteliales y células madre mesenquimales
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
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Sistema de ensayo de angiogénesis tridimensional utilizando esferoides de cocultura formados por células formadoras de colonias endoteliales y células madre mesenquimales

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9,478 Views

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09:24 min

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September 18, 2019

DOI:

10.3791/60032-v

DOI:
10.3791/60032-v

•

09:24 min
September 18, 2019

•

11 Views
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1College of Pharmacy, Duksung Innovative Drug Center,Duksung Women’s University, 2Korea Drug Development Platform using Radio-isotope,Korea Institute of Radiological & Medical Sciences

Summary

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El sistema de ensayo de angiogénesis esferoide cocultura tridimensional está diseñado para imitar la angiogénesis fisiológica. Los esferoides de cocultivo están formados por dos precursores de células vasculares humanas, EFCC y MSC, e incrustados en gel de colágeno. El nuevo sistema es eficaz para evaluar moduladores angiogénicos y proporciona información más relevante al estudio in vivo.

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Shah, S., Lee, H., Park, Y. H., Jeon, E., Chung, H. K., Lee, E. S., Shim, J. H., Kang, K. Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells. J. Vis. Exp. (151), e60032, doi:10.3791/60032 (2019).

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