/
/
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
9,478 Views
•
09:24 min
•
September 18, 2019
DOI:
Summary
Automatically generated
Three-dimensional co-culture spheroid angiogenesis assay system is designed to mimic the physiologic angiogenesis. Co-culture spheroids are formed by two human vascular cell precursors, ECFCs and MSCs, and embedded in collagen gel. The new system is effective for evaluating angiogenic modulators, and provides more relevant information to the in vivo study.
Explore More Videos
3D Co-culture SpheroidAngiogenesisEndothelial Colony Forming Cells (ECFCs)Mesenchymal Stem Cells (MSCs)Vascular Progenitor CellsCell InteractionsSproutingTubular MaturationPersonalized TreatmentsAbnormal Angiogenesis-related DiseasesCancerDrug DevelopmentTrypsin-EDTASingle Cell SuspensionPKH67PKH26Fluorescent DyeSpheroid Generation
Read Article
Cite this Article
Shah, S., Lee, H., Park, Y. H., Jeon, E., Chung, H. K., Lee, E. S., Shim, J. H., Kang, K. Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells. J. Vis. Exp. (151), e60032, doi:10.3791/60032 (2019).
Copy