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Genetics
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通过全基因组互惠性分析,对糖
母菌菌种间热耐性差异的遗传图谱
JoVE Journal
Genetics
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JoVE Journal
Genetics
Genetic Mapping of Thermotolerance Differences Between Species of
Saccharomyces
Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Please note that all translations are automatically generated.
Click here for the English version.
通过全基因组互惠性分析,对糖
母菌菌种间热耐性差异的遗传图谱
DOI:
10.3791/59972-v
•
10:08 min
•
August 12, 2019
•
Carly V. Weiss
2
,
Julie N. Chuong
,
Rachel B. Brem
3
1
Department of Plant and Microbial Biology
,
University of California Berkeley
,
2
Department of Biology
,
Stanford University
,
3
Buck Institute for Research on Aging
Chapters
00:04
Title
00:22
Preparation of Hybrid Yeast Cells for Transformation
01:55
Transformation of pJR487 into Hybrid Yeast Cells
08:16
Results: Thermotolerance Differences between Species of
Saccharomyces
09:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
通过测序(RH-seq)的互惠血性是绘制物种间特征差异遗传基础的有力新方法。血吸血池由转位子诱变产生,其适应性通过高全测序的竞争性增长进行跟踪。对结果数据的分析可确定特征背后的基因。
Tags
Keywords: Genetic Mapping
Thermotolerance
Saccharomyces Yeast
Genome-wide Reciprocal Hemizygosity Analysis
High-throughput
High Resolution
Freezer Stock
YPD Agar
Liquid YPD
Optical Density
Back Dilution
Tris-EDTA Buffer
Lithium Acetate
Polyethylene Glycol
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