Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Genetic Mapping of Thermotolerance Differences…
JoVE Journal
Genetics

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Genetics
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
 

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Article DOI: 10.3791/59972-v • 10:08 min • August 12th, 2019
August 12th, 2019 •
1,2, 3, 1,3
1Department of Plant and Microbial Biology, University of California Berkeley, 2Department of Biology, Stanford University, 3Buck Institute for Research on Aging

Capítulos

Resumen
Automatic Translation

Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.

Tags

Genetic Mapping Thermotolerance Species Saccharomyces Yeast Genome-wide Reciprocal Hemizygosity Analysis High-throughput High Resolution Applicable JR507 Freezer Stock Strain YPD Agar Plate Incubate Liquid YPD Glass Flask Od600 Measurement Back Dilute One Liter Flask Cultures
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter